Functional state of cardiomyocyte mitochondria in malignant process in presence of comorbid pathology in experiment

Purpose of the study. An analysis indices free radical oxidation and respiration mitochondria heart cells in a malignant process presence diabetes mellitus chronic neurogenic pain experimental animals. Materials methods. The study included outbred female rats (n=32) ?57?L/6 mice (n=84). Experimental groups were: intact group 1 (n=8), control (n=8) with (DM), comparison standard subcutaneous transplantation Guerin’s carcinoma, main carcinoma transplanted after week persistent hyperglycemia. 2 (n=21), (n=21) model (CNP), melanoma (B16/F10), (CNP+B16/F10) 3 weeks CNP creation. Heart were isolated by differential centrifugation. Levels cytochrome C (ng/mg protein), 8-hydroxy-2'-deoxyguanosine (8-OHdG) malondialdehyde (MDA) (?mol/g protein) measured mitochondrial samples ELISA. Statistical was performed using Statistica 10.0 program. Results. DM upregulated 8-OHdG 6.3 times MDA 1.9 (?=0.0000) downregulated 1.5 (?=0.0053) cell mitochondria, compared to values. DM+Guerin’s increased 14.0 1.7 decreased (?=0.0000), did not affect studied parameters heart. CNP+B16/F10 7.1 1.6 (?=0.0008). Conclusions. Comorbidity (diabetes mellitus, pain) together pathology aggravates dysfunction destabilization respiratory chain mediated processes.